Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

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چکیده

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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

BACKGROUND MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes. Due to the important biological role it is of great interest to quantitatively determine their expression level in different biological settings. RESULTS We describe a PCR method for quantification of microRNAs based on a single reverse tr...

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ژورنال

عنوان ژورنال: BMC Biotechnology

سال: 2011

ISSN: 1472-6750

DOI: 10.1186/1472-6750-11-70